0 ratings
зүйлийн дугаар:
125665119
20x SSPE Buffer 1 L
зүйлийн дугаар:
125665119
MNT 213157
Price Details
Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )
*All items will import from АНУ
0 ratings
Шүүмж бичих
Нөөцөд байна
USA дэлгүүрээс импортолсон
Яг одоо захиалаад ойрын хугацаанд хүлээн аваарай Баасан гараг, 7 дугаар сар 10
Secured transaction
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
Our Top Logistics Partners
20X SSPE is a prepared buffer concentrate for nucleic acid hybridizations and blot transfer applications.
Fast
Shipping
Free
Return*
Secure Packaging
100% Original Products
PCI DSS Compliance
ISO 27001 Certified
Note: Step Down Voltage Transformer required for using electronics products of АНУ store (110-120). Recommended power converters Одоо худалдаж авах.
Бүтээгдэхүүний дэлгэрэнгүй мэдээлэл
| Item Weight | 2.2 lbs (1 kg) |
БАРААНЫ ТАЙЛБАР
20x SSPE Buffer 1 L
Асуулга байна уу? Бидэнтэй чатлаарай
Хэрэглэгчийн асуулт ба хариултууд
-
Асуулт:
What is 20x SSPE Buffer and how is it used?
Хариулт: 20x SSPE Buffer is a high-concentration solution used primarily in molecular biology for various applications such as hybridization and washing of nucleic acid blots. This buffer provides an ideal ionic environment, enhancing the stability of DNA and RNA interactions during experiments. It's especially useful in procedures like Southern and Northern blotting, where high salt concentration helps maintain strand associations. By diluting it to a working concentration, researchers can optimize conditions for their experiments effectively. -
Асуулт:
What are the components of 20x SSPE Buffer?
Хариулт: 20x SSPE Buffer typically consists of sodium chloride, sodium phosphate, and EDTA. These components are crucial for maintaining the appropriate ionic strength and pH conditions during molecular biology applications. Sodium chloride stabilizes nucleic acid interactions, while sodium phosphate adjusts the pH, allowing for optimal hybridization conditions. EDTA chelates divalent metal ions that could otherwise degrade nucleic acids, ensuring sample integrity for accurate results. -
Асуулт:
How should 20x SSPE Buffer be stored?
Хариулт: 20x SSPE Buffer should be stored at a temperature of 4°C for short-term use and can be frozen at -20°C for long-term storage. When storing, it’s advisable to use airtight containers to prevent contamination and degradation from moisture and light. Regularly checking for any signs of precipitation or discoloration can ensure the buffer remains effective for experiments. Proper storage will help maintain its stability and reliability in various laboratory applications. -
Асуулт:
Can 20x SSPE Buffer be used for DNA gel electrophoresis?
Хариулт: While 20x SSPE Buffer is primarily designed for hybridization applications, it is not typically used as a running buffer for DNA gel electrophoresis. Instead, running buffers like TAE or TBE are preferred, as they provide better separation capabilities for nucleic acids. However, SSPE can be useful in post-electrophoresis applications, such as in southern blotting, to help maintain DNA stability during the transfer and hybridization processes. -
Асуулт:
What dilution is required to prepare a working solution from 20x SSPE Buffer?
Хариулт: To prepare a working solution from 20x SSPE Buffer, dilution to a 1x concentration is required. This means mixing one part of the 20x buffer with 19 parts of distilled water or an appropriate diluent. For example, to create 100 mL of 1x SSPE, you would add 5 mL of 20x buffer to 95 mL of water. This diluted solution can then be used in various downstream applications, enhancing the effectiveness of nucleic acid assays. -
Асуулт:
Is 20x SSPE Buffer suitable for use in RNA experiments?
Хариулт: Yes, 20x SSPE Buffer is suitable for RNA experiments and is particularly useful in applications such as RNA blotting and hybridization. Its high salt concentration helps maintain RNA stability and prevents the degradation of RNA during experimental procedures. In scenarios where high specificity in hybridization is essential, using 20x SSPE can enhance the signal and reduce background noise by providing optimal ionic conditions for RNA binding. -
Асуулт:
What role does EDTA play in 20x SSPE Buffer?
Хариулт: EDTA in 20x SSPE Buffer serves as a chelating agent that binds divalent metal ions like magnesium and calcium, which can catalyze the degradation of nucleic acids. By sequestering these ions, EDTA enhances the stability of both DNA and RNA during laboratory procedures, minimizing the risk of enzymatic breakdown. This is particularly important in applications like hybridization, where the integrity of nucleic acids is crucial for reliable results. -
Асуулт:
How can you utilize 20x SSPE Buffer in PCR applications?
Хариулт: While 20x SSPE Buffer is not typically used directly in PCR, it can be beneficial in the post-amplification steps, such as purification and probe hybridization. It provides a stable ionic environment for maintaining the integrity of PCR products. After amplification, using a 1x dilution of SSPE can help in the downstream applications, ensuring that the amplified fragments remain stable and intact during further analysis. -
Асуулт:
Where can I buy 20x SSPE Buffer 1 L in Mongolia?
Хариулт: You can buy 20x SSPE Buffer 1 L from Ubuy. They offer a variety of laboratory supplies, including this specific buffer, making it a reliable choice for your research needs in Mongolia.
Buffers Editorial Review
Редакцийн шүүмж олдсонгүй
Customer Reviews & Ratings
1 Хэрэглэгчийн үнэлгээ
-
5 од
100%
-
4 од
0%
-
3 од
0%
-
2 од
0%
-
1 од
0%
Энэ бараанд шүүмж өгөх
Бусад хэрэглэгчидтэй санал бодлоо хуваалцана уу
Product Price History
Чухал мэдээлэл
- Хязгаарлалт : Олон улсын хүргэлтээр илгээгдсэн барааны тохиолдолд, үйлдвэрлэгчийн баталгаа хүчингүй байх, үйлдвэрлэгчийн үйлчилгээ хүргэх боломжгүй, барааны заавар, гарын авлага болон анхааруулга очих улсын хэлдээр биш байх магадлалтай гэдгийг анхаарна уу. Мөн бараа болон дагалдах хэрэгсэл нь тухайн улсын стандартыг мөрдөөгүй, чанарын заалт болон шошгын шаардлагыг хангаагүй байх магадлалтай бөгөөд, тухайн улсын цахилгааны вольтод тохирохгүй байж болзошгүй (тохирох адаптер болон хувиргагч шаардлагатай) юм. Хүлээн авагч нь барааг тухайн улс уруу хуулийн дагуу импортлогдохыг батлах ёстой. Ubuy болон түүний холбоот түншүүдээс худалдан авсан тохиолдолд худалдан авагч нь хүлээн авах улсын бүх хууль болон заалтуудыг мөрдөх ёстой.
- Ubuy нь дэлхий даяар хэрэглэгддэг хайлтын хэрэгсэл учраас Ubuy дээр байгаа бараа бүр зарагдана гэсэн үг биш. Бараанууд нь экспорт болон худалдааны журмыг дагаж мөрдөх ёстой.
MNT 213157
Яг одоо захиалаад ойрын хугацаанд хүлээн аваарай Баасан гараг, 7 дугаар сар 10
This item is not restrict in my country.(Please click on above link if this item is not restrict in your country, So our team will review and allow.)
QTY:
Secured transaction
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
Our Top Logistics Partners
Fastest cross-border delivery
Онцлог & Давуу талууд
- Ready-to-use 20X SSPE buffer concentrate for ease of use.
- Ideal for nucleic acid hybridizations and blotting.
- Liquid form ensures convenience in application.
- Composed of essential components: NaCl, NaH2PO4, EDTA.
- Stable at room temperature for easy storage and handling.
- High concentration offering effective results in gel electrophoresis and blotting.