50X TAE Buffer (Tris-Acetate-EDTA), 100 mL
MNT 161363
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50X TAE Buffer is used for running electrophoresis of nucleic acids in gels, providing electrical conductivity and maintaining pH, while protecting DNA from nucleases.
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Бүтээгдэхүүний дэлгэрэнгүй мэдээлэл
| Item Weight | 0.22 lbs (100 grams) |
Who Should Buy?
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Molecular Biologists
Ideal for DNA electrophoresis, providing efficient buffering capacity for nucleic acid analysis in lab experiments.
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Academic Institutions
Supports educational labs where students learn about genetic analysis, making it a staple in biology curricula.
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Research Facilities
Highly suitable for ongoing research projects involving DNA and RNA studies, thanks to its reliable buffering properties.
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Non-Laboratory Use
Not designed for applications outside of laboratory settings; unsuitable for general household or consumer use.
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Basic Science Classes
May be too specialized for introductory classes where simpler methods or buffers may suffice for educational purposes.
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Field Work
Not practical for outdoor experiments or fieldwork, where environmental conditions can affect the buffer's efficacy.
БАРААНЫ ТАЙЛБАР
50X TAE Buffer (Tris-Acetate-EDTA), 100 mL
Хэрэглэгчийн асуулт ба хариултууд
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Асуулт:
What is 50X TAE Buffer Tris-Acetate-EDTA used for?
Хариулт: 50X TAE Buffer Tris-Acetate-EDTA is primarily used in molecular biology for nucleic acid electrophoresis. This concentrated buffer allows for efficient separation of DNA and RNA in gel electrophoresis techniques. When diluted, it maintains the pH and ionic environment necessary for accurate results during DNA analysis, cloning, and other genetic applications. -
Асуулт:
How do you prepare 1X TAE Buffer from 50X concentration?
Хариулт: To prepare 1X TAE Buffer from 50X concentration, dilute the buffer by mixing 1 part of 50X TAE with 49 parts of distilled water. This results in a final volume where the buffer is at the appropriate dilution for running gel electrophoresis, ensuring the components are balanced for optimal performance in your experiments. -
Асуулт:
Why is Tris-Acetate-EDTA preferred over other buffers?
Хариулт: Tris-Acetate-EDTA (TAE) is favored for its ability to provide consistent buffering capacity and low electroendosmosis during electrophoresis. Unlike other buffers, TAE allows for the recovery of nucleic acids post-electrophoresis as it does not interfere with downstream applications, making it ideal for both analysis and cloning of DNA. -
Асуулт:
Can TAE Buffer be reused after gel electrophoresis?
Хариулт: Generally, TAE Buffer may be reused if it remains clean and free from contaminants. However, repeated use can compromise the buffer's pH and ionic strength, affecting the results of subsequent experiments. Always evaluate the quality of the buffer after each use and consider preparing fresh buffer for critical applications to maintain accuracy. -
Асуулт:
What are the storage conditions for 50X TAE Buffer?
Хариулт: 50X TAE Buffer should be stored in a cool, dark place, ideally at room temperature or in a refrigerator to maintain its efficacy. Properly sealing the bottle after use will prevent contamination. If stored correctly, it can remain stable for an extended period, ensuring it's ready for your experimental needs. -
Асуулт:
Is 50X TAE Buffer compatible with all types of gel electrophoresis?
Хариулт: Yes, 50X TAE Buffer is compatible with various gel electrophoresis methods, including agarose and polyacrylamide gels. While TAE is typically used for DNA applications, it's important to adapt your experiments based on the specific type of nucleic acids being analyzed to achieve the best results. -
Асуулт:
How does the concentration of TAE Buffer impact electrophoresis results?
Хариулт: The concentration of TAE Buffer used directly impacts the resolution and migration speed of nucleic acids during electrophoresis. Higher concentrations can lead to slower migration and better resolution in separating fragments, while lower concentrations may generate faster migration, making it necessary to optimize based on the experiment's needs. -
Асуулт:
What pH does 50X TAE Buffer maintain?
Хариулт: 50X TAE Buffer typically maintains a pH around 8.0, which is optimal for most enzymatic reactions and electrophoresis processes. This pH ensures stability and functionality, providing a conducive environment for nucleic acid interactions and separation during experimental procedures in molecular biology. -
Асуулт:
Can I use 50X TAE Buffer for RNA electrophoresis?
Хариулт: Yes, 50X TAE Buffer can be used for RNA electrophoresis, although many prefer using 1X TBE for RNA as it offers sharper bands. However, TAE still effectively provides a stable environment for RNA separation, making it a suitable choice in many laboratory applications involving RNA. -
Асуулт:
Where can I buy 50X TAE Buffer Tris-Acetate-EDTA, 100 mL in Mongolia?
Хариулт: You can purchase 50X TAE Buffer Tris-Acetate-EDTA, 100 mL from Ubuy, which offers a wide selection of laboratory supplies that can be delivered to your location in Mongolia. Ubuy ensures that you receive a product that meets your experimental requirements.
eConLabSupply Buffers Editorial Review
Customer Reviews & Ratings
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Бусад хэрэглэгчидтэй санал бодлоо хуваалцана уу
Давуу тал
- High-quality buffer solution
- Stable and reliable performance
- Easy to use in experiments
- Excellent for electrophoresis
- Great customer reviews
Сул талууд
- Some may find the size too small.
Product Price History
Чухал мэдээлэл
- Хязгаарлалт : Олон улсын хүргэлтээр илгээгдсэн барааны тохиолдолд, үйлдвэрлэгчийн баталгаа хүчингүй байх, үйлдвэрлэгчийн үйлчилгээ хүргэх боломжгүй, барааны заавар, гарын авлага болон анхааруулга очих улсын хэлдээр биш байх магадлалтай гэдгийг анхаарна уу. Мөн бараа болон дагалдах хэрэгсэл нь тухайн улсын стандартыг мөрдөөгүй, чанарын заалт болон шошгын шаардлагыг хангаагүй байх магадлалтай бөгөөд, тухайн улсын цахилгааны вольтод тохирохгүй байж болзошгүй (тохирох адаптер болон хувиргагч шаардлагатай) юм. Хүлээн авагч нь барааг тухайн улс уруу хуулийн дагуу импортлогдохыг батлах ёстой. Ubuy болон түүний холбоот түншүүдээс худалдан авсан тохиолдолд худалдан авагч нь хүлээн авах улсын бүх хууль болон заалтуудыг мөрдөх ёстой.
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MNT 161363
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Онцлог & Давуу талууд
- Ideal for running electrophoresis of nucleic acids
- Provides electrical conductivity and maintains pH
- Protects DNA from nucleases
- Suitable for both genomic and large supercoiled DNA
- Can be used as a gel preparation buffer